Ub-Rhodamine 110
Ub-Rhodamine 110 is a quenched, fluorescent substrate for deubiquitinating enzymes. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110 results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).
Product Name:
Ub-Rhodamine 110
>Also Known As:
N/A
>Catalog No.:
M3020
>Size:
50 µg
>Molecular Weight:
8936 Da by MS
>Species:
Human
>Source:
Synthetic
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
N/A
>Quality Assurance:
≥95% by RP-HPLC and SDS-PAGE
>Storage:
Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: Ub-Rhodamine 110 |
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Activity assay of Ub-Rhodamine 110 (100 nM) with varying concentrations UCH-L3 (5 – 313 pM). |
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Reaction buffer: 20 mM Tris, pH 7.1 at 37°C, 150 mM NaCl, 2 mM DTT. Reactions contained 0.5 μM Ub-Rhodamine 110 alone (control, brown line), or with 10 nM GST-Usp15 (gray line) or with 20 nM GST-Usp15 (yellow line). Rhodamine110 fluorescence was monitored by using a plate reader with excitation/emission filters at 485/20 and 530/30 nm, respectively.
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>Shipping Method:
Dry ice shipping
>References:
1. A. Tirat et al., (2005) Anal. Biochem., 343, 244-255.
2. U. Hassiepin et al., (2007) Anal. Biochem., 371, 201-207. SLx-2119 18469826
