O excite the bases within the DNA strand itself and observe the energy transfer to a fluorophore. Our most recent fluorescent nucleotide analog, Furano-dT, allows one to do just that. Upon unwinding of a hybridized strand, a fivefold increase in fluorescence is observed at 470 nm when exciting at 260 nm. However, when exciting at 350 nm, no hybridization dependence is seen, which should allow the determination of relative population states. Also, because this fluorophore has only two additional carbon atoms, it may be well tolerated by DNA binding proteins.
PRODUCT UPDATE – WHICH CHEMICAL PHOSPHORYLATION REAGENT
So why do we need to be offering two chemical phosphorylation reagents The original chemical phosphorylation reagent (CPR) (10-1900) works wonderfully giving the 5′-phosphate in virtually quantitative yield. Unfortunately though, it is not compatible with DMT-on purification techniques since the sulfonylethyl group is eliminated in ammonium hydroxide regardless of the presence of the DMT group. Enter CPR II. Coupling efficiency with CPR II (10-1901) is exactly the same as with the original version. However, now you have a choice. Remove the DMT group on the synthesizer and the ammonium hydroxide step generates the 5’phosphate. Or now you can leave the DMT on and simply purify the oligo using a reverse phase cartridge or HPLC. We are happy to report that CPR II has been awarded US Patent No. 5,959,090 which issued on September 28, 1999.
Item Chemical Phosphorylation Reagent Chemical Phosphorylation Reagent II (CPR II) Catalog No. 10-1900-90 10-1900-02 10-1901-90 10-1901-02 Pack 100 ole 0.25g 100 ole 0.25g Price($) 50.00 160.00 60.00 200.00
GLEN RESEARCH ON THE WORLD WIDE WEB Visit Glen Research at http://glenres. Find what’s new, obtain catalog numbers, and access technical support information.
C-5 PROPYNES, CYTOFECTIN AND OTHER PRODUCT UPDATES
C-5 Propynes, Cytofectin GSV
In The Glen Report, 1993, 6(2), 1, we introduced the C-5 Propynyl Pyrimidine derivatives to the research community, under license from Gilead Sciences, Inc. Oligos containing these modifications have proved to be attractive for antisense experiments because of their enhanced binding to their purine partners. At that time, we noted that these properties would also prove to be useful in primers and probes and, in recent years, the majority of uses seem to have been in more routine biological experiments.779353-01-4 IUPAC Name In The Glen Report, 1996, 9(1), 1, we introduced Cytofectin GSV, a transfection reagent, designed at and licensed by Gilead, to be used to transport antisense oligonucleotides into cells even in the presence of serum.2922-83-0 InChIKey Although optimal for the transfection of antisense oligonucleotides, Cytofectin GSV has also been found to be suitable for plasmid transfection.PMID:31298506 In December 1998, Gilead’s antisense technology, including the patents covering the C-5 Propynes and Cytofectin GSV, was purchased by Isis Pharmaceuticals, Inc. In March, 2000, Isis terminated our license to continue to manufacture and supply these products, effective July 9, 2000. Glen Research and Isis have been unable to reach a new agreement to allow Glen to continue supplying these compounds in the long term. Further supply of these products is covered by our original contract with Gilead and we may continue to supply inventory existing on July 9, 2000 for a further 12 months, or until the supply is depleted. We sincerely regret that our association with these products will be ending and, especially, we reg.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
