Mammalian SWI/SNF complexes are composed of either BRM or BRG1 and 9�C12 additional subunits, which are referred as BRM- or BRG1-associated factors called BAFs. Individual SWI/SNF complexes contain either BRM or BRG1, but not both, such that BRM/BAF complexes are structural distinct from BRG1/BAF complexes. Beside their ATPase subunit, the chromatin remodeling complexes differ in the composition of associated cofactors, which stimulate and modulate qualitatively the remodeling activity within the complex. In addition, the associating subunits are believed to mediate targeting of the ATPase subunit to integrate nucleosome remodeling into a physiological context. Mammalian SWI/SNF complexes are involved in the dynamic transcriptional regulation of a large array of genes including cell cycle regulators, signaling proteins, genes regulating the architecture of the cell and adhesion to the extra cellular matrix. In addition, SWI/SNF complexes are critical mediators of RB and p53 to induce cell cycle arrest and are required for BRCA-mediated DNA repair, pointing toward a fundamental function of these proteins as tumor suppressors. BRM knockout mice as well as BRG1 heterozygous mice are more prone for cancer development. Analysis of human tumor samples has revealed that BRG1 and BRM are coordinately silenced in various human cancers indicating that silencing of BRG1 and/or BRM could be an important step in the etiology of a significant number and diverse range of human tumors has been recently characterized as a MCE Chemical 315706-13-9 protein that inhibits the invasive migration of human tumor cells through the control of MAL/SRF signaling. To gain further insight into how SCAI impacts on gene transcription, we have performed a screen for SCAI-interacting proteins. We show here that SCAI is functionally (-)-Methyl rocaglate linked to SWI/ SNF complexes to promote changes in gene expression that may be critical for tumor cell invasion. SCAI is a transcriptional repressor of SRF and a highly conserved protein among vertebrates. The protein expression of SCAI in native human tissues and its sub-cellular localization have not yet been determined. Here, we investigated levels of protein expres
