Curcuminoid-3. (TIF)Determine S3 The ESI-MSMS spectra fragmentation pattern of curcumin and mitocurcuminoids. A, Curcumin (m z 369; CE = 35 eV); B, Mitocurcuminoid-1 (mz 487; CE = 35 eV); C, Mitocurcuminoid-2 (mz 671; CE = 35 eV); D, Mitocurcuminoid3(mz457;CE = forty eV). (TIF) Determine S4 ESI-MS spectra of curcumin (ten mM) treated MCF -7 cells. A, cytosolic fraction and B, mitochondrial fraction. Insert demonstrates the calibration curve of mitocurcuminoid-1 (ten mM) addressed MCF-7 cells: cytosolic fraction and mitochondrial fraction. Price similar to every place is an ordinary of triplicate measurements designed on distinct times. The mistake bars inside the determine signify the regular deviations from three triplicate measurements. (TIF) Determine S5 ESI-MS spectra of mitocurcuminod-1 (10 mM) treated MCF-7 cells. A, cytosolic fraction and B, mitochondrial portion. . Insert displays the calibration curve of Mitocurcuminoid-2 (10 mM) addressed MCF cells: cytosolic fraction and mitochondrial fraction. Price equivalent to each and every place is surely an common of triplicate measurements designed on distinctive days. The mistake bars inside the figure depict the standard deviations from three triplicate measurements. (TIF) Determine S6 ESI-MS spectra of mitocurcuminoid-2 (10 mM) taken care of MCF-7 cells. A, cytosolic fraction and B, mitochondrial fraction. Insert reveals the calibration curve of mitocurcuminoid-3 (10 mM) taken care of MCF cells: cytosolic fraction and mitochondrial portion. Price corresponding to every single point can be an normal of triplicate measurements made on distinct times. The error bars during the figure symbolize the normal deviations from a few triplicate measurements. (TIF) Determine S7 ESI-MS spectra of mitocurcuminoid-3 (ten mM) addressed MCF cells. A, cytosolic fraction and B, mitochondrial fraction. Insert shows the calibration curve of curcumin (ten mM) addressed MCF cells: cytosolic portion. Benefit similar to each and every level is undoubtedly an typical of triplicate measurements created on distinct times. The mistake bars during the determine signify the normal deviations from 3 triplicate measurements. (TIF)Mitochondrial-Targeted CurcuminoidsFigure S8 Cell Viability of MCF-10A dealt with withvarious concentrations of curcumin and mitocurcuminoids. MCF-10A cells ended up incubated with various concentrations of curcumin and mitocurcuminoids-1, two and three for 24 h. Within the conclude of treatment plans, cell viability was assayed by SRB assay. (TIF)Figure S9 Mobile Viability of MCF-10A treated withof TPP for 24 h. Within the finish of treatment plans, cell viability was assayed by SRB assay. (TIF)Writer ContributionsConceived and designed the experiments: Auto SGK VS TG VKK. Executed the experiments: Car or truck VS AKK SK KR. Analyzed the data: Car or truck SGK SP NV VKK. Wrote the paper: Vehicle SGK SP PK VKK.many concertrations of triphenylphosphonium (TPP). MCF-7 cells were incubated with numerous concentrations
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