As applied, the stronger the impact of corn residue on lowering N2 O emissions. Combining the application of corn stalks and urea could reduce the concentration of NH4 + -N and NO3 – -N derived from urea, and then lessen the substrate essential for N2 O production in nitrification and denitrification processes. Additionally, the combined application of corn stalks and urea could efficiently inhibit the abundance of essential N2 O-producing genes AOA amoA, nirS and nirK. Keyword phrases: N2 O; corn stalks; urea; 15 N isotope; ammonium nitrogen; nitrate nitrogen1. Introduction Mitigating damaging international climate alter caused by greenhouse gas (GHG) emissions is one of the significant challenges in sustainable development [1,2]. Nitrous oxide (N2 O) will be the third largest greenhouse gas [3], having a greenhouse effect 298 times greater than that of CO2 on a 100-year scale [4], along with a substantial contributor to the destruction on the stratospheric ozone [5]. Agricultural soil will be the key supply of N2 O [8] and contributes roughly 60 of global anthropogenic N2 O emissions [9]. For that reason, a complete understanding of N2 O emission from agricultural soils is crucial for the formulation of reasonable emission reduction approaches. Nevertheless, most studies on N2 O emissions from agricultural soils have been conducted in temperate or humid ecosystems exactly where water and nutrients are certainly not scarce, while you can find somewhat Leukotriene D4 Cancer couple of research on N2 O production in arid locations [4,10]. As certainly one of the world’s largest agricultural countries, China produces 21 from the world’s corn [11]. Liaoning Province is certainly one of China’s 13 principal grain-producing regions, as well as the semi-arid location of northwestern Liaoning accounts for greater than 2/3 of corn Decanoyl-L-carnitine web cultivation within this province [12]. This extensive region of cultivation is also an comprehensive location of N2 O production. Therefore, exploring the processes related with N2 O production in corn fields in semi-arid northwestern Liaoning has important sensible significance for farmlandPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Copyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This short article is definitely an open access report distributed under the terms and conditions of the Inventive Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ four.0/).Agronomy 2021, 11, 2009. https://doi.org/10.3390/agronomyhttps://www.mdpi.com/journal/agronomyAgronomy 2021, 11,two ofgreenhouse gas emission reduction. N2 O is made primarily by microbial nitrification and denitrification processes, among which AOA amoA and AOB amoA are the important genes of N2 O production inside the nitrification pathway, and nirS and nirK would be the key genes of N2 O production within the denitrification pathway [4]. The determination of those genes assists us to far better have an understanding of the pathway of N2 O production. Application of nitrogen fertilizer may be the main explanation for the enhance in N2 O emissions from farmland [135]. Nonetheless, the application of nitrogen fertilizer is definitely an significant measure to make sure meals safety, so it truly is not feasible to lessen N2 O emissions from farmland merely by reducing the level of nitrogen fertilizer [16]. In order to combat an growing atmospheric N2 O concentration, other N2 O mitigation techniques are needed, certainly one of which is to minimize N2 O emissions in farmland soil by altering soil properties by means of the return of corn stalk residue [17]. Corn is planted extensively in northwes.
