Rythrocytes, as exposure of red blood cells to as much as 100 M p4 for two h did not result in hemolysis (Fig. 2C). Likewise, human key keratinocytes did not considerably alter their mitochondrial respiration in response to higher doses (12.500 M) of p4 at 2 h, as assessed by 3-(four,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell viability assay (Fig. S1). Equivalent data had been obtained when IL-10R2 Proteins Recombinant Proteins release of intracellular enzyme lactate dehydrogenase in to the conditioned medium was used as a marker of keratinocyte cytotoxicity, though, at the highest dose (one
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