iersin.RelA/p65 Species orgAugust 2021 | Volume 12 | ArticleTorres-Silva et al.De novo Transcriptome of M. glaucescens Shoot OrganogenesisFIGURE six | Networks of differentially expressed genes. The networks had been developed making use of GO categories in BiNGO for M. glaucescens explants just before (manage) and after (treated) shoot organogenesis induction. Bubble size and colour indicate the frequency of your GO term and the P-value, respectively.Frontiers in Plant Science | frontiersin.orgAugust 2021 | Volume 12 | ArticleTorres-Silva et al.De novo Transcriptome of M. glaucescens Shoot OrganogenesisTABLE 3 | Transcription aspect households containing downregulated and upregulated mGluR2 list unigenes following M. glaucescens shoot organogenesis induction. Transcription element family Domain No. of unigenes Downregulated Upregulated AP2 AP2 zf-Dof DUF260 B3 and auxin response B3 zf-LSD1 G2-like GRAS Homeobox Homeobox zf-CCCH NAM bZIP_1 WRKY Myb_DNA-binding Myb_DNA-binding HLH EIN3 HSF_DNA-bind zf-C2H2 Trihelix GATA 2 1 0 5 1 0 two 2 1 0 four 0 3 1 four 0 1 0 four 0 1 3 0 0 0 0 0 1 3 1 0 0 0 1 0 1 1 5 1 7 0 four 11 five two two three three 1 5 three 17 5 two 10 four four three two 7 8 five 2 two 1 1 0 1 0 1 1 1 1 1 0 0 0ERF household protein AP2 loved ones protein Dof family protein LBD household protein ARF loved ones protein B3 household protein LSD family protein G2-like household protein GRAS household protein HB-other loved ones protein TALE family members protein C3H household protein NAC family members protein bZIP loved ones protein WRKY household protein MYB family members protein MYB_related household protein bHLH family members protein EIL loved ones protein HSF family protein C2H2 household protein Trihelix household protein GATA loved ones proteinHD-ZIP family protein HomeoboxE2F/DP household protein E2F_TDP CAMTA family members protein CG-1 NF-YC household protein NF-YA family members protein NF-X1 family members protein ZF-HD family members protein SBP loved ones protein YABBY loved ones protein FAR1 household protein MIKC_MADS family members protein GRF family members protein S1Fa-like loved ones protein TCP loved ones protein DBB loved ones protein NF-YC CBFB_NFYA zf-NF-X1 ZF-HD_dimer SBP YABBY FAR1 SRF-TF and K-box WRC and QLQ S1FA TCP zf-B_boxCO-like family protein zf-B_box and CCTdivision and cell wall formation in the course of regeneration (Bao et al., 2009). Additionally, changes in cell fate can happen because of a new balance involving euchromatin and heterochromatin following dynamic alterations in chromatin structure (Zhao et al., 2008), indicated here by the presenceof histone-encoding genes among the upregulated transcripts (Figure 5). Transcription factor households and unigenes have been extra abundant among the upregulated than downregulated unigenes (Table three). This acquiring indicates that many metabolic pathways are activated during shoot organogenesis induction. The families of transcription things discovered to become upregulated within this study (i.e., NF-Y, MYB, ERF, E2F, LBD, and NAC) are akin to those identified through plant organogenesis, additional confirming the function of those regulators in vegetative regeneration (Cheong et al., 2002; Bao et al., 2009; Cervantes-P ez et al., 2018; Nadiya et al., 2018; Pal et al., 2018; Xi et al., 2019). During the in vitro culture preceding shoot organogenesis induction, the metabolism of M. glaucescens was characterized by fewer upregulated than downregulated genes, corroborating the low growth rate standard of cacti cultures (Lema-Rumiska and n Kulus, 2014; P ez-Molphe-Balch et al., 2015). Downregulated genes were largely involved in key metabolism. They integrated transcripts related to photosynthesis (15) and antenna proteins (five) identified in KEGG
