Name:
Enterokinase Protein
Synonyms:
rBoEnterokinase, His; Enteropeptidase; ENTK; PRSS7,重组肠激酶
Species Name:
Bovine
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
E6Y432
Gene Id:
Uniprot E6Y432, with C-His TagMIVGGSDSREGAWPWVVALYFDDQQVCGASLVSRDWLVSAAHCVYGRNMEPSKWKAVLGLHMASNLTSPQIETRLIDQIVINPHYNKRRKDNDIAMMHLEMKVNYTDYIQPICLPEENQVFSPGRICSIAGWGTLIYQGSTADVLQEADVPLLSNEKCQQQMPEYNITENMVCAGYEAGGVDSCQGDSGGPLMCQENNRWLLAGVTSFGYQCALPNRPGVYARVPRFTEWIQSFLHHHHHHH
Molecular Weight:
40 kDa(Reducing)
Purity:
>80% by SDS-PAGE
Physical Appearance Name:
Liquid
Endotoxin Name:
<0.2EU/μg
Reconstitution:
Stability Storage:
· 12 months from date of receipt, -20 to -70 °C as supplied. · 6 months, -20 to -70 °C under sterile conditions after reconstitution.· 1 week, 2 to 8 °C under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Buffer System:
20mM Tris-HCl, pH7.2, 200mM NaCl, 2mM CaCl, 50% Glycerol
Quality Statement:
Enterokinase (enteropeptidase, EC 3.4.4.8) occupies a key position in the utilization of dietary proteins. The enzyme initiates intraluminar digestion of proteins by the proteolytic conversion of trypsinogen to trypsin, which in turn activates the other pancreatic zymogens (Kunitz, 1939a,b; Hadorn et al., 1969). The proteolytic attack of enterokinase is directed exclusively toward the Lys6-Ile7 peptide bond of trypsinogen leaving all other lysine and arginine bonds in the molecule unaffected (Maroux et al., 1971). The resultant cleavage produces the simultaneous release of active trypsin and of the amino-terminal hexapeptide Val-(Asp)d-Lys (Rovery et al., 1953; Davie and Neurath, 1955). This unique specificity exhibited by enterokinase is of interest as it relates to both the molecular basis of substrate recognition and the control of the digestive process.
Reference:
Anderson LE, Walsh KA, Neurath H. Bovine enterokinase. Purification, specificity, and some molecular properties. Biochemistry. 1977 Jul 26;16(15):3354-60. doi: 10.1021/bi00634a011. PMID: 889800.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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