6xHis-GST-TRABID(NZF1)
TRABID is an OUT domain-containing deubiquitinating enzyme that exhibits specificity to K29/K33-linked polyubiquitin chains. 6xHis-GST-TRABID(NZF1) contains the N-terminal NZF1 domain (amino acid 1-33) of TRABID fused at the C-terminal of the 6xHis-GST tag. TRABID(NZF1) specifically binds K29/K33 polyubiquitin chains. This protein can be used to pull down K29 and K33 polyubiquitin chains using Ni-NTA resin or glutathione resin.
Product Name:
6xHis-GST-TRABID(NZF1)
>Also Known As:
NZF1
>Catalog No.:
I1810
>Size:
100 µg
>Molecular Weight:
29 kDa (with tags)
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~85% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
 |
|
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-GST-TRABID(NZF1)
|
>Shipping Method:
Dry ice shipping
>References:
Michel MA, et al. (2015) Mol Cell. 58(1):95-109 BPTES 18483622
