6xHis-TAB2 (NZF)
6xHis was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro pulldown assays and for enrichment of cellular proteins conjugated with lysine 63 polyubiquitin chains in whole cell or tissue lysates. 6xHis-TAB2 (NZF) can be precipitated using Nickel resin. After washing, 6xHis-TAB2 (NZF) and its bound proteins can be eluted by a buffer containing 200 mM imidazole.
Product Name:
6xHis-TAB2 (NZF)
>Also Known As:
CHTD2; MAP3K7IP2
>Catalog No.:
I1710
>Size:
100 µg
>Molecular Weight:
7.7 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~85% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
NM_015093.4
>Image(s):
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-TAB2 (NZF) |
>Shipping Method:
Dry ice shipping
>References:
1. van Wijk SJ, et al. (2013) Nat Protoc. 8(7):1449-58
2. van Wijk SJ, et al. (2012) Mol Cell. 14;47(5):797-809 Dapagliflozin 18483441
