GST-NEMO (UBAN)
GST was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO en-compassing amino acids 183-339. This fusion protein can be used for in vitro GST pulldown as-says and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. GST-NEMO (UBAN) can be precipitated using glutathione resin. After washing, GST-NEMO (UBAN)and its bound proteins can be eluted by a buffer containing 10 mM glutathione.
Product Name:
GST-NEMO (UBAN)
>Also Known As:
IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma
>Catalog No.:
I1530
>Size:
100 µg
>Molecular Weight:
18.4 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~90% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
NM_001099857
>Image(s):
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified GST-NEMO (UBAN) |
>Shipping Method:
Dry ice shipping
>References:
1. van Wijk SJ, et al. (2013) Nat Protoc. 8(7):1449-58
2. van Wijk SJ, et al. (2012) Mol Cell. 14;47(5):797-809 Panobinostat 18483349
