GST-S5a (UIM)
GST was fused in the N-terminus of the two UIM domains of S5a, which specifically bind K48-linked polyubiquitin chains. This fusion protein can be used for enrichment of K48-linked polyubiquitinated proteins in cell or tissue lysates, and for in vitro GST pulldown assays.
Product Name:
GST-S5a (UIM)
>Also Known As:
S5a-UIM
>Catalog No.:
I1620
>Size:
100 µg
>Molecular Weight:
11.3 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~85% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified GST-S5a (UIM) |
>Shipping Method:
Dry ice shipping
>References:
1. Chiariotti L, et al. (1998) Genomics 47(2), 258 – 265.
2. Fedele M, et al. (2000) J Biol Chem 275(11), 7894 – 7901.
3. Hunter T, Sun H (2008) Ernst Schering Found Sump Proc (1), 1 – 16.
4. Tatham MH, et al. (2008) Nat Cell BIol 10(5), 538 – 546.
5. Bruderer R, et al. (2011) EMBO Rep 12(2), 142 – 148. LY-411575 18483401
